In this paper, we formulate a regular -fractional Sturm–Liouville problem (qFSLP) which includes the left-sided Riemann–Liouville and the right-sided Caputo q-fractional derivatives of the same order , . The properties of the eigenvalues and the eigenfunctions are investigated. A -fractional version of the Wronskian is defined and its relation to the simplicity of the eigenfunctions is verified. We use the fixed point theorem to introduce a sufficient condition on eigenvalues for the existence and uniqueness of the associated eigenfunctions when . These results are a generalization of the integer regular -Sturm–Liouville problem introduced by Annaby and Mansour in[1]. An example for a qFSLP whose eigenfunctions are little -Jacobi polynomials is introduced.

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