Let be a connected complex semi-simple Lie group, and let be an -dimensional Bott-Samelson variety of , where is any sequence of simple reflections in the Weyl group of . We study the Poisson structure on defined by a standard multiplicative Poisson structure on . We explicitly express on each of the affine coordinate charts, one for every subexpression of , in terms of the root strings and the structure constants of the Lie algebra of . We show that the restriction of to each affine coordinate chart gives rise to a Poisson structure on the polynomial algebra ${}[z_1, \ldots, z_n]G$. For canonically chosen , we show that the induced Poisson structure on for every affine coordinate chart is in fact defined over , thus giving rise to an iterated Poisson Ore extension of any field of arbitrary characteristic. The special case of on the affine chart corresponding to the full subexpression of ${\bf u}$ yields an explicit formula for the standard Poisson structures on {\it generalized Bruhat cells} in Bott-Samelson coordinates. The paper establishes the foundation on generalized Bruhat cells and sets up the stage for their applications, some of which are discussed in the Introduction of the paper.

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