Let and be finite partially ordered sets with , and and $(Q) \subset ^dPQ\Gamma((P),(Q)) \subset ^d(P) \cup (-(Q))$. It is known that twinned chain polytopes are Gorenstein Fano polytopes with the integer decomposition property. In the present paper, we study combinatorial properties of twinned chain polytopes. First, we will give the formula of the volume of twinned chain polytopes in terms of the underlying partially ordered sets. Second, we will identify the facet-supporting hyperplanes of twinned chain polytopes in terms of the underlying partially ordered sets. Finally, we will provide the vertex representations of the dual polytopes of twinned chain polytopes.

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