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We consider the off-shell formulation of the 5D, =1 super Yang-Mills and super Chern-Simons theories in harmonic superspace. Using such a formulation we develop a manifestly supersymmetric and gauge invariant approach to constructing the one-loop effective action both in super Yang-Mills and super Chern-Simons models. On the base of this approach we compute the leading low-energy quantum contribution to the effective action on the Abelian vector multiplet background. This contribution corresponds to invariant which is given in 5D superfield form.

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