Let be a sequence of real projective bundles such that $M_i\to M_{i-1}i=1,2,\ldots,n$, is a projective bundle of a Whitney sum of a real line bundle and the trivial line bundle over . The above sequence is called the real Bott tower and the top manifold is called the real Bott manifold. There are a few ways to decide whether there exists a Spin-structure on an oriented flat manifold . An oriented flat manifold has a Spin-structure if and only if there exists a homomorphism such that , where is the covering map. There is an equivalent condition for existence of Spin-structure. This is well known that the closed oriented differential manifold has a Spin-structure if and only if the second Stiefel-Whitney class vanishes. Our paper is a sequel of A. G\k{a}sior, A. Szczepa'nski, Flat manifolds with holonomy group of diagonal type, Osaka J. Math. 51 (2014), 1015 - 1025. There are given non-complete conditions of the existence of Spin-structures on real Bott manifolds. In this paper, if k is even, we formulate necessary and sufficient conditions of the existence of Spin-structure on real Bott manifolds. Here is our main result The real Bott manifold has a Spin-structure if and only for all $1\leq i<j\leq nM(A_{ij})A_{ij}n\times ni-j-$th nonzero rows.

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