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We present phase shift measurements for neutron matter waves in vacuum and in low pressure Helium using a method originally developed for neutron scattering length measurements in neutron interferometry. We search for phase shifts associated with a coupling to scalar fields. We set stringent limits for a scalar chameleon field, a prominent quintessence dark energy candidate. We find that the coupling constant is less than 1.9 ~for at 95% confidence level, where is an input parameter of the self–interaction of the chameleon field inversely proportional to .

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