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For any knot which bounds non-orientable and null-homologous surfaces in punctured , we construct a lower bound of the first Betti number of which consists of the signature of and the Heegaard Floer -invariant of the integer homology sphere obtained by -surgery along . By using this lower bound, we prove that for any integer , a certain knot cannot bound any surface which satisfies the above conditions and whose first Betti number is less than .

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