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The three-dimensional jump conditions for the pressure and velocity fields, up to the second normal derivative,across an incompressible/inextensible interface in the Stokes regime are derived herein. The fluid viscosity is only piecewise continuous in the domain while the embedded interface exerts singular forces on the surround fluids. This gives rise to discontinuous solutions in the pressure and velocity field. These jump conditions are required to develop accurate numerical methods, such as the Immersed Interface Method, for the solutions of the Stokes equations in such situations.

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