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We study the twisted N=4 super Yang-Mills theories in the Omega-background with the constant R-symmetry Wilson line gauge field. Based on the classification of topological twists of N=4 supersymmetry (the half, the Vafa-Witten and the Marcus twists), we construct the deformed off-shell supersymmetry associated with the scalar supercharges for these twists. We find that the Omega-deformed action is written in the exact form with respect to the scalar supercharges as in the undeformed case.

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