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We study the real roots of the Yablonskii-Vorob’ev polynomials, which are special polynomials used to represent rational solutions of the second Painlev'e equation. It has been conjectured that the number of real roots of the nth Yablonskii-Vorob’ev polynomial equals [(n+1)/2]. We prove this conjecture using an interlacing property between the roots of the Yablonskii-Vorob’ev polynomials. Furthermore we determine precisely the number of negative and the number of positive real roots of the nth Yablonskii-Vorob’ev polynomial.

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