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We have calculated low-lying quasiparticle excitation spectra of rotating three-dimensional Bose-Einstein condensates. As opposed to the prediction of hydrodynamic continuum theories, we find a minimum in the Tkachenko mode spectrum at intermediate rotation frequencies of the harmonic trap. Such a minimum can harbour a Tkachenko quasiparticle with zero excitation energy. We discuss the experimental signatures of such a zero mode.

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