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The paper [Phys. Lett. A 373 (2009) 3610] by D.-C. Li analyzes the transformation between two-qubit mixed states by local operations and classical communication. We show that the proof of the main theorem, Theorem 2.6 in [Phys. Lett. A 373 (2009) 3610] is not complete. Therefore the generalization of Nielsen’s theorem to mixed states still remains an open problem.

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