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In recent articles, the investigation of atomic bases in cluster algebras associated to affine quivers led the second-named author to introduce a variety called transverse quiver Grassmannian and the first-named and third-named authors to consider the smooth loci of quiver Grassmannians. In this paper, we prove that, for any affine quiver Q, the transverse quiver Grassmannian of an indecomposable representation M is the set of points N in the quiver Grassmannian of M such that Ext^1(N,M/N)=0. As a corollary we prove that the transverse quiver Grassmannian coincides with the smooth locus of the irreducible components of minimal dimension in the quiver Grassmannian.

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