Although relativistic heavy-ion collisions have emerged as a powerful probe for studying nuclear structure, the potential influence of nucleon-nucleon short-range correlations (NN-SRCs) on the initial state has remained an open question. By incorporating NN-SRCs into the initial conditions, we demonstrate that higher-order fluctuations of the initial transverse size, -particle , which can be directly mapped to final-state mean transverse momentum fluctuations, exhibit remarkable sensitivity to NN-SRCs. Quantitatively, and differ by more than 10% between systems with and without NN correlations. Moreover, we report a universal scaling of these quantities with and the average nuclear density, mirroring the connection between the SRC effect and the EMC effect in electron scatterings. This work establishes relativistic heavy-ion collisions as a new tool for probing nuclear structure and constraining two-body or many-body NN interactions across different energy scales and system sizes.

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