Thermodynamics of two-flavor QCD at finite temperature and density is studied on a lattice, using a renormalization group improved gauge action and the clover improved Wilson quark action. In the simulations along lines of constant , we calculate the Taylor expansion coefficients of the heavy-quark free energy with respect to the quark chemical potential () up to the second order. By comparing the expansion coefficients of the free energies between quark()and antiquark(), and between and , we find a characteristic difference at finite due to the first order coefficient of the Taylor expansion. We also calculate the quark number and isospin susceptibilities, and find that the second order coefficient of the quark number susceptibility shows enhancement around the pseudo-critical temperature.

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