We present a dual-region experimental apparatus for trapping single atoms of the fermionic isotope ytterbium-171 () with optical tweezers in a science chamber containing an in-vacuum nanofiber cavity. A source region is located approximately from the science chamber, preserving optical access around the chamber for high-numerical-aperture tweezer optics. A pulsed transport sequence based on longitudinal moving molasses launches atomic pulses toward the science chamber, where they are decelerated and recaptured in a magneto-optical trap. We subsequently load single atoms into a tweezer array located approximately from the nanofiber and measure a lifetime of . These results demonstrate that the dual-region architecture provides sufficient atoms for single-atom tweezer operation near an in-vacuum nanofiber cavity.

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