Observations of the 21-cm signal can probe energy injection into the intergalactic medium arising from dark matter annihilation during cosmic dawn and reionization. For s-wave annihilation, the annihilation rate depends on the squared dark matter density, making the 21-cm signal sensitive to dark matter halo substructure. In this work, we extend the numerical method of DM21cm to address spatially inhomogeneous s-wave annihilation including subhalo effects, using semi-analytical treatment of SASHIMI-C. Our numerical results show that the projected HERA limit for the annihilation channel is stronger than the Leo~T bound within the dark matter mass range of , whereas the projected HERA limit for the annihilation channel is the strongest among the existing bounds for –.

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