Let be the adjacency spectral center of a tree , and let be its characteristic set. We determine the largest possible separation among trees of every order . Writing , we prove , and . The argument rests on a simple opposition between two rooted-tree weights. An endpoint-rooted path minimizes adjacency spectral radius, but maximizes bottleneck Perron value. A one-sided replacement by a path therefore cannot decrease the distance between the two centers. Quantitatively, this gives the sharp estimate . A preliminary six-vertex barrier shows that disjoint center sets require at least twelve vertices, and the four-leaf broom is extremal for every .

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